What is Full process kinetics-PCR?
Full Process Kinetics-PCR (FPK-PCR) is a novel approach to real time PCR data analysis, specifically developed to overcome the limitations the classical analysis tools which assume the reaction efficiency to be constant. .It is a powerful tool capable of estimating the initial reaction efficiency (Emax) and its cycle-to-cycle changes as well as the initial amount of target sequences (i0).
What can FPK-PCR do for you?
Real-time PCR analysis has become a key tool in molecular biology. Yet, despite its general use and marked improvements in thermocycler hardware over the past decade, little has changed in the way real time PCR data are typically analyzed. Most current approaches assume constant efficiency over a single reaction. Such methodology performs well as long as PCR efficiency does not significantly vary between reactions. Yet, small inaccuracies in reaction efficiency can lead to large quantification errors. Especially when working with biological samples, the possible presence of coextracted inhibitors poses a challenge to data analysis.
FPK-PCR allows the user to:
- Estimate reaction efficiency (Emax)
- Directly estimate the initial number of target copies
- Display the complete evolution of cycle efficiencies throughout the reaction
- Calculate the Cq value
- Display graphical output
More text & comparison with other methods
History
FPK-PCR was developed within the...
Ghent University
Ghent University, abbreviated to UGent, is one of the major universities in the Dutch-speaking region of Europe. It distinguishes itself as a socially committed and pluralistic university in a broad international perspective..
Scientific Institute of Public Health
The Belgian Scientific Institute for Public Health (known as WIV-ISP) provides support for public health policy through scientific research, expert opinions and divisional tasks.
