VIB-RUG Department of Molecular Biomedical Research

Molecular ImmunoBiotechnology Unit

 

 

 

 

 

 

 

 

Up Promoter Translation Fusion Repression Secretion mRNA stab

more on ExpressX...

bulletThe ExpressX system includes a variety of expression strategies, all brought toghether into a uniform vector design.  This allows for rapid screening of strategies to be further developed. Strategies include:
bulletCytoplasmic expression of mature protein.
bulletSecretion to the periplasm, using different elements.
bulletThe use of a variety of fusion proteins, cleavable with a variety of restricted proteases.
bulletThe vector frame is optimized for efficient translation initiation and mRNA stability.
bulletAn optimized promoter and induction strategy was developed.
bulletA collection of host strains can be used.

ExpressX is created to facilitate expression studies in E. coli. This technology is available through collaboration.

If you feel this is interesting for you, please contact Dr. Nico Mertens of the VIB1 - DMBR Department.

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